Abstract

The synergistic efficacy of the aqueous extract of combined garlic and clove (CGC) was studied against a single garlic aqueous extract (GE) administration in trona-induced osteopontin inflammation using male Wistar rats (180-200 g) within 35 days. Group 1 received standard animal chow and water ad libitum, and group 2 received 200 mg/kg body weight of trona (TRN). Group 3 was given 200 mg/kg body weight of trona but co-treated with 100 mg/kg body weight of CGC; group 4 was co-administered with 200 mg/kg body weight of trona and 100 mg/kg body weight of garlic extract; group 5: 200 mg/kg body weight of trona and 150 mg/kg body weight of CGC. In contrast, 6 was given 200 mg/kg body weight of trona and 150 mg/kg body weight of garlic extract. The assault revealed a significant (p<0.05) increase in the plasma levels of osteopontin (15-fold), alanine transaminase (ALT) (2.6-fold), and a corresponding significant (p < 0.05) decrease in the concentration of reduced glutathione (GSH) (2.7-fold) in the agonist assaulted group when compared with the animal on standard chow. However, the disorders were ameliorated by the synergetic potential of CGC more than the single application of GE in a dose-dependent manner, revealing a significant and more synergetic recuperation in the co-treated animals. The study further revealed significant histo-hepatic remediation by CGC more than GE, as microscopically depicted in the photomicrographs of the fibrotic lesion induced by TRN on the parenchymal tissue of the treated rats’ hepatocytes. More so, the result was able to affirm the folkloric use and direct simultaneous increase/great correlation of the novel osteopontin, a proteinous cytokine, and the inflammatory hepatic bio-indicator, ALT. Hence, it’s established that CGC could be considered a good herbal therapeutic candidate for hematological and hepatic-embedded osteopontin regeneration.